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Detailed classification and selection of experimental centrifuges
Author: BIOLAB Published: 2015-5-8 Visits: 957

The experimental centrifuge is mainly selected from two aspects of speed and capacity according to the workload. The following describes the characteristics of the experimental centrifuge in detail:

(1) Speed: Centrifuges are divided into low speed centrifuges (<1 0000 rpm / min), high speed centrifuges (1 0000 rpm / min to 30,000 rpm / min), and ultra high speed centrifuges (> 30000 rpm) according to the maximum speed / Min), each centrifuge has a rated maximum speed. The maximum speed refers to the speed under no-load conditions, but the maximum speed varies depending on the type of rotor and the size of the sample. For example: The rated speed of a centrifuge is 16000 rpm / min, which means that the rotor rotates 16,000 times per minute at no load. After adding the sample, the speed must be less than 16000 rpm / min. Different rotors have different maximum speeds (an imported centrifuge can be equipped with multiple rotors). The horizontal rotor can reach 5000 rpm / min, but the angular rotor can reach approximately 14000 rpm / min. Please consult the product sales for specific differences Personnel and relevant technical personnel of the production plant, so be careful in selecting the speed, and the maximum speed of the selected centrifuge should be higher than the target speed. For example, if the target speed is 16000 rpm / mIn, the maximum speed of the selected centrifuge must be higher than 16000 rpm / min.

(2) Temperature: Some samples (such as proteins, cells, etc.) will be destroyed in high temperature environments. This requires a refrigerated centrifuge, which has a rated temperature range. When the centrifuge is running at high speed, the heat generated by the centrifuge and the refrigeration system of the centrifuge are balanced at a certain temperature (generally, the samples for freezing and centrifugation need to be maintained at 3 ° C to 8 ° C). How much can be achieved is also related to the rotor. For example, a centrifuge The rated temperature range is -10 ℃ ~ 60 ℃. When the horizontal rotor is installed, it can reach about 3 ℃ when rotating. If it is an angular rotor, it may only reach about 7 ℃. This point should also consult the product sales staff and the relevant technical staff of the production plant in detail.

(3) Capacity: How many sample tubes need to be centrifuged each time 7 How much capacity is needed for each sample tube 7 These factors determine the total capacity of a centrifuge, simply put the total capacity of the centrifuge = the capacity of each centrifuge tube × centrifugation The number of tubes, total capacity and workload are matched.

(4) Rotor: The rotor of the centrifuge is mainly divided into two types, the horizontal rotor: the hanging blue is in a horizontal state during operation, at right angles to the shaft, and the sample concentrates the sediment at the bottom of the centrifuge tube: the angle rotor: the centrifuge container and the shaft are fixed together Angle, the sample concentrates the sediment on the bottom of the centrifuge tube and the side wall near the bottom. If you want the sample to be concentrated on the bottom of the centrifuge tube, select the horizontal rotor. If you want to concentrate the sample on the bottom of the centrifuge tube and on the side wall near the bottom, select the angle rotor. There are also some special tests or special samples that require special rotors such as: large-capacity hanging baskets (mostly used in blood stations), microplate reader rotors, slide rotors, PCR rotors, test tube rack rotors, and capillary rotors. The rotors have fixed specifications, which are combined with the capacity of the centrifuge, such as an angle rotor of 36 × 5 ml, which determines both the type of rotor and the capacity of the centrifuge, so the selection of the rotor is very important.

(5) Control system: Microcomputer control systems are used in high-end centrifuges. These control systems can not only ensure the safe operation of the centrifuge, but also complete the work tasks automatically. Many centrifuges now have better humanized control systems. For example: rotor identification function, safety lock function, fault prompt function, acceleration and deceleration curves, etc. In addition to the above points, pay attention to some details and necessary accessories. The main part of the centrifuge is a motor. The motor is divided into a carbon brush motor and a carbon brushless motor. The former has been eliminated. Most of the centrifuges are carbon brushless motors, and some motors also have a brake function. Refrigeration centrifuges are also different in terms of refrigeration, and now the environmentally friendly technology is of course fluorine-free refrigeration. In addition to considering the noise problem, try to choose a quieter centrifuge to maintain a comfortable experimental environment. Also be cautious in terms of accessories. Some experiments use special centrifuge tubes (centrifuging toxic samples or samples requiring ultra-high speed centrifugation). Such centrifuge tubes must be equipped with corresponding sleeves to be safer. There are also some special sample containers (irregular sample bottles, blood bags, etc.). These details and accessories must be carefully considered when choosing a centrifuge, otherwise normal work cannot be performed.

It can be inferred from the above selections that the low-speed centrifuge is better than the high-speed centrifuge in temperature control, or the capacity may be larger. If you have any questions, please consult the Shanghai Boshang centrifuge hotline: 021-31262808

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